Experimental measurements of the steady forces on a central cluster of tubes in a rotated triangular array subjected to two-phase air–water cross-flow have been conducted. The tests were done for a series of void fractions and a Reynolds number (based on the pitch velocity), The forces obtained and their derivatives with respect to the static streamwise displacement of the central tube in the cluster were then used to perform a quasi-steady fluidelastic instability analysis. The predicted instability velocities were found to be in good agreement with the dynamic stability tests. Since the effect of the time delay was ignored, the analysis confirmed the predominance of the stiffness-controlled mechanism in causing streamwise fluidelastic instability. The effect of frequency detuning on the streamwise fluidelastic instability threshold was also explored. It was found that frequency detuning has, in general, a stabilizing effect. However, for a large initial variance in a population of frequencies (e.g., ), a smaller sample drawn from the larger population may have lower or higher variance resulting in a large scatter in possible values of the stability constant, some even lower than the average (tuned) case. Frequency detuning clearly has important implications for streamwise fluidelastic instability in the steam generator U-bend region where in-plane boundary conditions, due to preload and contact friction variance, are poorly defined. The present analysis has, in particular, demonstrated the potential of the quasi-steady model in predicting streamwise fluidelastic instability threshold in tube arrays subjected to two-phase cross-flows.